37%ofallcellculturesupernatants,andalmostallofconfluentcellculturesupernatants,inhibitPCR.ProficiencystudieshavedemonstratedtheimportanceoffurtherDNAextractionforasensitivePCRresultsevenforunremarkablesamplematerials.
TheVenor®GeMSamplePreparationKitprovidessampleshighlycompatIBLewithMinervaBiolabsVenor®GeMMycoplasmaPCRkits.AspecialprotocolallowsthepurificationofthesamplesinordertomeetthehighdemandsoftheEP2.6.7.ThesampleDNAiselutedinavolumeof50µlinahighconcentrationandpurityformaximumsensitivityofthePCR.ThekitworkswithDNAextractioncolumnswhicharedirectlysuitableforthetreatmentofupto250µlofyoursample.
ScopeofApplication
ForDNAextractionfrom:
Cellculturesupernatantsincludingcellsandcelldebris
ATMPs
Sampleswithpolymersandglycerine(e.g.cryoconservationmedia)
Cellculturemedia
Proteinsolutions,incl.antibodiesandenzymes.
KitComponents
Spincolumns
Collectiontubes
Conditioner,Washandelutionbuffers
RequiredConsumables
Ethanolisrequiredforthereconstitutionofthewashbufferbutnotincludedinthekit.Microcentrifugevials(1.5ml)areneededtoDNAelution.
Atproteinconcentrationsabove10mg/mlinthesamplepretreatmentwithProteinaseKisstronglyrecommended(Cat.-No.11-9905).
PackageSizes
- Cat.No.56-101010extractions
- Cat.No.56-105050extractions
- Cat.No.56-1200200extractions
Storage
Storeandshipmentatambienttemperature.
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