Venor®GeMClassicallowsfast,reliableandtime-savingroutinemonitoringofmycoplasmacontaminationbyPCR.

TypeofPCR

Forconventional,endpointPCR

RecommendedUse/Scope

ApplicableinresearchandindustryfordirecttestingofcellculturesandBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnostics.

KitComponents

Lyophilizedprimersetsandnucleotides
10xreactionbufferoptimizedforMBTaqDNAPolymerase
LyophilizedpositivecontrolDNA
Lyophilizedinternalamplificationcontrol

PackageSizes

Primersetsandnucleotidesarepreparedinaliquotsof25tests.

• Cat.No.11-102525Tests
  Cat.No.11-105050Tests
  Cat.No.11-1100100Tests
  Cat.No.11-1250250Tests

Resultevaluation

GelelectrophoresisatendpointofPCR

RequiredConsumables

Polymerase.Wehighlyrecommendourreliablehot-startMBTaqDNAPolymerase,Cat.-No.53-0050/100/200/250.
PCRreactiontubes

OptionalforprocessvalidationandEP2.6.7complianttesting:
InternalControlDNAextra(4vialsfor300µleachofinternalamplificationcontrol;Cat.No.11-1905)
10CFU™SensitivityStandardsavailableforallEPlistedmycoplasmaspecies

Requiredlabdevices

RegularPCRcycler
AgarosegelelectrophoresisandDNAstainingsystem
Pipettingequipment
Tubecentrifuge

ShelfLifeandStorage

Componentsaremaintainableat+2to+8°Cforatleast6months.Afterrehydratisationthereagentsmustbestoredat-18°C.

EP2.6.7compliance

UseofVenor®GeMClassicforQAtestingofbiologicalslikemasterandworkingcellbanks,autologouscells,culturemedia,bulkharvestandfinalproducttestingaccordingtoEP2.6.7isapplicableafterappropriatesamplepreparationandprocessvalidation.

Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.