Venor®GeMAdvancecontainsPCRreactiontubespre-coatedwithallPCRreagentstoreducethetotalassaytimewithoutneedtopreparealiquotsofamastermix.ForadditionalconveniencethegelloADIngbufferanddyearealreadyincludedinthereactionbuffer.AfterthermalcyclingthePCRcanbeloadeddirectlyontheagarosegel.

TypeofPCR

Forconventional,endpointPCR

ScopeofApplication

Applicableinresearchfordirecttestingofcellculturesandcellculture-derivedBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnosticsortestingofhumansamples.

KitComponents

Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledin0.2mlPCRreactiontubes
Rehydrationbufferincludinggelloadingbufferandrunningdye
Positivecontrols,lyophilized,filledin0.2mlPCRreactiontubes
PCRgradewater

PackageSizes

• Cat.No.11-702424Tests
  Cat.No.11-704848Tests
  Cat.No.11-709696Tests
  Cat.No.11-7240240Tests

Resultevaluation

GelelectrophoresisatendpointofPCR
RequiredConsumables

AgaroseandDNA-specificdyeforgelstaining

Requiredlabdevices

Regularblock-heatedPCRcycler
Agarosegelelectrophoresisandgeldocumentationsystem
Pipettingequipment
Tubecentrifuge

ShelfLifeandStorage

Kitcomponentsaremaintainableat+2to+8°Cforatleast6months.AfterrehydratisationoftheindividualPCRtubesthereagentsmustbeusedwithin1hour.

EP2.6.7compliance

No

Fig.AmplifiedPCRproductsarevisualizedbystandardgelelectrophoresis.