Venor®GeMqOneStepisamycoplasmaqPCRdetectionkitwhichincludesallreagentsrequiredfortheqPCRreaction.Primer,nucleotides,polymeraseandtheinternalamplificationcontrolareprovidedready-to-useinalyophilizedreactionmix.RehydrationbufferandlyophilizedPositiveControlDNAarealsoprovidedinthekit.The assaycanbeperformedwithanytypeofreal-timePCRcyclerabletodetectthefluorescencedyesFAM™andHEX™.Theprotocolprovidedis recommendedforthefastandreliablescreeningofcellculturesupernatants.Thedetectionprocedurecanbeperformedwithin3hours.

MycoplasmasarespecificallydetectedbyamplifyingahighlyconservedrRNAoperon,ormoreprecisely,the16SrRNAcodingregioninthemycoplasmagenome.Themycoplasma-specificamplificationisdetectedat520nm(FAM™channel).The qOneStepPCRreactionmix includesprimersetsandFAM™labeledprobeswhichallowthespecificdetectionofallMollicutespeciessofardescribedascontaminantsofcellculturesandmediacomponents.EukaryoticDNAisnotamplifiedbythisprimer/probesystem.FalsenegativeresultsduetoPCRinhibitorsorimproperDNAextractionaredetectedbytheinternalamplificationcontrolwhich isalready includedinqOneStepreactionmix.Theamplificationoftheinternalamplificationcontrolisdetectedat610nm(HEX™channel).

TypeofPCR

TaqMan®based qPCRAssaywithFAM™andHEX™labeledprobes

ScopeofApplication

Applicableinresearchand developmentfordirecttestingofcellculturesandBIOLOGicals.Intendedforresearchuseonly.Notrecommendedforclinicaldiagnosticsortestingofhumansamples.

KitComponents

Primersets,nucleotides,internalamplificationcontrolandpolymeraseatoptimizedconcentrations,lyophilizedandpre-castinonetube
RehydrationBuffer
LyophilizedPositiveControlDNA
PCRgradewater

PackageSizes

qOneStepPCRreactionmixes arepreparedinaliquotsof25reactionseach.

• Cat.No.11-9102525tests
  Cat.No.11-91100100tests
  Cat.No.11-91250250tests

Resultevaluation

cycler-based,real-timePCR

RequiredConsumables

PCRreactiontubes

Requiredlabdevices

qPCRcycler
Pipettingequipment
Microcentrifuge

ShelfLifeandStorage