ConviFlex™DNAmpMixisalyophilizedready-to-usePCRmastermix,whichconsistsofallcorecomponentsrequiredforPCRamplification,exceptDNAtemplateandprimers.

PCRamplificationhasbecomeanindispensablemethodineverymolecularBIOLOGylab.Usually,thecommonreactioncomponents(e.g.buffer,dNTPs,MgCl₂,andDNApolymerase)aremixedtogetherbylaboperatorstocreateamastermix,whichisthenaliquotedtoindividualreactiontubes.Reaction-specificprimersandtemplateDNAareaddedpriortoPCR,addingfurtherpipettingstepstotheprocedure,andthusincreasingthemarginoferror,theriskofcross-contaminationsandworktime.TheapplicationofConviFlex™DNAmpMixrequiresminimalhands-ontimeandreducestheriskofcross-contaminationsduetotheminimalnumberofpipettingstepsrequiredtosetupthePCRreaction.

RecommendedUse/Scope

ConviFlex™DNAmpMixiscompatIBLewithbothconventionalPCRandqPCR,andissuitableforallregularPCRapplicationsincludingPCRassayswithcomplexgenomicorCDNAtemplates,lowcopynumbertargets,highspecificityPCR,T/Acloning,andmultiplexPCR.DNAtemplatecanbeextractedfromdiversestartingmaterialslikecellculturesupernatants,bacterialcolonies,andmammaliancellsandtissues.

ConviFlex™DNAmpMixisalsosuitableforPCRamplificationusingtemplateDNAdirectlyfromcellculturesupernatantsorbacterialsUSPensions.

KitComponents

ConviFlex™DNAmpMixcontainshotstartTaqpolymeraseanddNTPs(100reactions/vial),RehydrationBuffer,MgCl₂(100mM)

PackageSizes

• Cat.No.191-0100100reactions
• Cat.No.191-0500500reactions
• Cat.No.191-10001000reactions

RequiredConsumables

Microcentrifugevials(1.5ml),Pipetteswithcorrespondingfiltertips,PCRtubes

RequiredLabDevices

Microcentrifuge,Heatblock,PCRThermocycler

ShelfLifeandStorage

LyophilizedConviFlex™DNAmpMixandRehydrationBuffershouldbestoredat2–8°C.Afterrehydration,ConviFlex™DNAmpMixshouldbestoredat<-18°Candisstableevenafterseveralfreeze/thawcycles.