SwabUp™LabMonitoringPluskitwithacontamination-freePCRsystemisfortracingDNAcontaminationhot-spotsinmolecularBIOLOGylabsinordertoefficientlyeliminatethemandpreventfutureoccurrence.

Smallamountsofamplicon-ortargetDNAcontaminationscouldleadtoPCRartifactsandfalsepositiveresults.Originatingfromaerosolizedfragmentsincentrifuges,Pipettesandotherlabequipmentorfromsmallsplattersduringworkingwithopenreactiontubes,contaminantDNAisveryhardtoremoveandcanleadtocrosscontaminationsbetweensamplesandwidespreadproblemsthroughoutthetestingprocedureandinterpretationofresults.SwabUp™LabMonitoringPluskitcontainsasamplecollectionsystem,aDNAextractionsystem,whichwasoptimizedfortheefficientdetectionofthesmallestamountsofcontaminantDNA,andacontamination-freePCRSystem.

RecommendedUse/Scope

ThiskitisrecommendedfortheregularmonitoringofthelabworkareaanddetectionoftargetorampliconDNAcontaminations,butespeciallyforsevereandpersistentcasesofDNAcontamination,whereeverysinglereagentusedforPCRcouldpotentiallybecontaminatedmakingitextremelydifficulttotracethecontaminationsource.

SwabUp™LabMonitoringPluskitintroducesthecontamination-freeSwabUp™DNAmpMix,auniversalPCRmastermix,whichcontainsallPCRcorecomponents.Onlyreaction-specificprimersandtemplateDNAareaddedbytheuser.

SwabUp™LabMonitoringkitisforresearchuseonly.ItisnotrecommendedforclinicalanddiagnosticapplicationsorforthedetectionofRNAcontaminations.

KitComponents

Separatelypackedswabs,CollectionBuffertubes,spincolumn-basedDNAextractionsystem,SwabUp™DNAmpMix,RehydrationBuffer,MgCl₂.

PackageSizes

• Cat.No.182-001010applications(25PCRreactions)
• Cat.No.182-005050applications(125PCRreactions)

RequiredConsumables

Ethanol(>96%abs.),1.5mlreactiontubes,PCRtubes

RequiredLabDevices

Microcentrifuge,heatblock,pipettingequipment,PCRThermocycler

ShelfLifeandStorage

DNAextractionsystemshouldbestoredatroomtemperature,CollectionBuffertubesmustbestoredat2–8°C.Swabscanbestoredat2–30°C.SwabUp™DNAmpMixshouldbestoredat2–8°C,andafterrehydrationat<-18°C.