OurPCRQuantificationStandardscontaingenomicDNAextractedfromlowpassageofdefinedmicroorganisms.TheDNAstartingmaterialwasanalyzedwithstandardizedmethodstodeterminetheDNA’sintegrityandquantity.OnlypureandintactDNAshowinga>10kbbandinanagarosegelandanOD260/280>1.8isused.TheDNAwaspartiallysequencedtoconfirmthespecies’identity.

ScopeofApplication

PCRQuantificationStandardscontainadefinednumberofgenomecopiesofaparticularmicroorganism.Therefore,ourstandardsareperfectlysuitableforgeneratingDNAstandardcurvesinqPCR.Inaddition,standardsarerequiredfortestingthespecificityandsensitivityofPCRassays,particularly,inthecontextofqualitycontrolandincorrespondencewithEP2.6.7.compliance

KitComponents

1vialwith1×10⁸genomes(freeze-dried.)
3vialswithTrisbuffer(10mM,pH8.5)

AvailableSpecies

• Cat.No.52-0116Acholeplasmalaidlawii
• Cat.No.52-5571Bordetellapertussis
• Cat.No.52-0083Escherichiacoli
• Cat.No.52-0101Legionellapneumophila
• Cat.No.52-0129Mycoplasmaarginini
• Cat.No.52-0117Mycoplasmafermentans
• Cat.No.52-0115Mycoplasmagallisepticum
• Cat.No.52-0130Mycoplasmahyorhinis
• Cat.No.52-0112Mycoplasmaorale
• Cat.No.52-0119Mycoplasmapneumoniae
• Cat.No.52-0124Mycoplasmasynoviae
• Cat.No.52-0164Spiroplasmacitri
• Cat.No.52-0071Pseudomonasaeruginosa

Storage

Thekitmustbestoredat2-8°C(Shippedatambienttemperatures).