MeatID™HalalisakitforthefastandreliableidentificationofporkanddonkeyinmeatandotherfoodsbyqPCR.

Theidentificationofdifferentmeats,especiallyinmincedmeatproductsisaserioustaskinfoodsafetyandfromandethicalperspective,especiallyforMuslims.Authenticationofforbiddenornonedeclaredingredientssuchasporkordonkeymeatisessentialtoensureconfidenceinthesupplychainandregulatorycompliance.

MeatID™Halalisavailableforrapidandreliableanalysisfromvariousmatricesincludingraw,orevenhighlyprocessedandcookedmeatproductswheretheDNAmaybesignificantlydegraded.ItispossIBLetoidentifyrelevantspeciesdowntoathresholdlevelof0.5%withasemi-quantitativeresult.

TypeofPCR

TaqMan®AssaybasedonFAM™andHEX™labeledprobes.

RecommendedUse/Scope

CanbeusedinresearchandindustryfordetectionofporkanddonkeyinmeatandotherfoodsbyqPCR.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.

Targetsequenceisamitochondrialgene(cytochromeb),whichisavailableinmultiplecopiespercell,enablingsuccessfuldetectionofminuteamountsoftargetDNA!

PackageSizes

• Cat.No.12-02-02525tests
  Cat.No.12-02-100100Tests

ResultEvaluation

Cyclerbased,real-timePCR

Sensitivity

Downtoathresholdlevelof0.5%withasemi-quantitativeresult.

Content

Primersets,nucleotides,polymeraseandinternalcontrolDNA,lyophilized,filledinasingletube.Rehydrationbuffer,Positivecontrol,PCRgradewater.

SampleRequirements

DNAcanbeextractedfromsamplematerialusingDNAextractionkitse.g.ExtractNow™DNAMiniKit(Art.-Nr.608-1050)orusermethod.

RequiredConsumables

Pipettetips,1.5mlreactiontubes,PCRreactiontubes

RequiredLabDevices

qPCRcyclerwithFAM™andHEX™filter,benchtopcentrifuge,racksfortubes,variablemicroliterpipettes

Timetoresult

Approx.90minutes

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationreagentsmustbestoredat-18°C.