FoodControl™qPCRisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviareal-timePCR.
DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™qPCRisadiagnosticsystemforeasydeterminationofcontaminationdegreeinagriculturalorfoodindustryviareal-timePCR.
TypeofPCR
TaqMan®AssaybasedonFAM™andHEX™labeledprobes.
RecommendedUse/Scope
Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
Thekitisavailableforthedetectionofthefollowingmicroorganismsviasemi-quantitaivereal-timeorconventionalPCR:
| Micro-organism | Target |
| Salmonellaenterica | invasionproteinA(invA)gen |
| Yersiniaenterocolitica | heatstableenterotoxinAgen |
| Shigellaspp. | invasionplasmidantigen(ipaH6)gen |
| Campylobacterspp. | acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamineO-acyltransferase(lpxA)gen |
| Clostridiumperfringens | phospholipaseCalphatoxin(plc)gen |
| Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin1(stx1)gen |
| Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin2(stx2)gen |
| EscherichiacoliO157 | wBDRgen |
| EscherichiacoliO104 | wckDgen |
| Listeriaspp. | invasionassociatedproteinp60(iap)gen |
| Listeriamonocytogenes | listeriolysinO(hly)gen |
| Salmonellaspp. | spacer-regionbetween16Sand23SRNAgenes |
PackageSizes
Packagesize25reactionseach:
- Cat.No.11-02-01-025Salmonellaenterica
- Cat.No.11-02-02-025Yersiniaenterocolitica
- Cat.No.11-02-03-025Shigellaspp.
- Cat.No.11-02-04-025Campylobacterspp.
- Cat.No.11-02-05-025Clostridiumperfringens
- Cat.No.11-02-06-025ShigaToxin1
- Cat.No.11-02-07-025ShigaToxin2
- Cat.No.11-02-08-025EscherichiacoliO157
- Cat.No.11-02-09-025EscherichiacoliO104
- Cat.No.11-02-10-025Listeriaspp.
- Cat.No.11-02-11-025Listeriamonocytogenes
- Cat.No.11-02-12-025Salmonellaspp.
ResultEvaluation
Cyclerbased,real-timePCR
Sensitivity
Downto10DNAcopies/assay.
Content
qPCRMix,SpeciesMix,RehydrationBuffer,PCRGradeWater,InternalControl,PositiveControl
SampleRequirements
IsolatedtotalDNAfrompotentiallycontaminatedfoodserveshereasstartingmaterial,typicallyafterpre-cultivationofthesamplegrowthmedium.
RequiredConsumables
PCRreactiontubes,1.5mlreactiontubes
Optional:DNAcanbeextractedfromenrichmentbrothusingtheDNAextractionkitExtractNow™FoodControl(Cat.No.609-1050)orusermethod.
RequiredLabDevices
qPCRcyclerwithFAM™andROX™filter
VariablemicroliterPipettes
Benchtopcentrifugefor1.5mlreactiontubes
Timetoresult
150minutes
ShelfLifeandStorage
Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydrationthereagentsmustbestoredat-18°C.
Compliance
No,certificationinprogress
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