FoodControl™LFAisaPCR-kitforfastandreliabledetectionoffoodbornepathogensviaconventionalPCRincombinationwithourreliableLateralFlowAssay(LFA)evaluationtechnology.
DetectingbacterialcontaminationisakeyaspectinfoodmicroBIOLOGy.Consequently,microbiologicalqualitycontrolprogramsareintensifiedthroughoutfoodchainproductioninordertominimizetheriskofintoxicatingconsumers.ReliABIlity,speedandrobustnessofatestsystemtodetectthepresenceorabsence,oreventhedegreeofcontaminationwithpathogens,becomesincreasinglyimportant.FoodControl™LFAisadiagnosticsystemforeasynon-quantitativedetectionofpresenceorabsenceoffoodbornepathogensinagriculturalorfoodindustryviaconventionalPCRandLateralFlowAssay(LFA)evaluationtechnology.
TypeofAssay
TheassayisbasedonaonetubeamplificationwithaconventionalPCRcyclerandasubsequentprobespecifichybridizationtechnique.Theresultinginterpretationisasimplereadoutofcolouredbarsonalateralflowstripe.
RecommendedUse/Scope
Canbeusedinresearchandindustryfordetectionofpathogeniccontaminationinfoodoragriculturalproducts.Forresearchuseonly!Donotuseforclinicaldiagnosticortestingofpatientsamples.
ThekitisavailableforthedetectionofthefollowingmicroorganismsviaconventionalPCR:
Features
| Micro-organism | Target |
| Salmonellaenterica | invasionproteinA(invA)gen |
| Yersiniaenterocolitica | heatstableenterotoxinAgen |
| Shigellaspp. | invasionplasmidantigen(ipaH6)gen |
| Campylobacterspp. | acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine O-acyltransferase(lpxA)gen |
| Clostridiumperfringens | phospholipaseCalphatoxin(plc)gen |
| Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin1(stx1)gen |
| Shigelladysenteriae, Escherichiacoli(EHEC) | ShigaToxin2(stx2)gen |
| EscherichiacoliO157 | wBDRgen |
| EscherichiacoliO104 | wckDgen |
| Listeriaspp. | invasionassociatedproteinp60(iap)gen |
| Listeriamonocytogenes | listeriolysinO(hly)gen |
| Salmonellaspp. | spacer-regionbetween16Sand23SRNAgenes |
