Thenumberofvegetariansandpeoplefollowingtheveganlifestyleincreasesfromyeartoyear.Thismaybeduetoethical,religious,culturalorhealthreasons.Howdoyouensurethatyourvegan-declaredfood has really beenprocessedwithoutmeat oranimalderivedproducts?

The VeganControl™kit,aqPCRbasedsystemforthedetectionofanimaltracesinfood,isaquickandinexpensivesolutiontothisProblem.

TypeofPCR

TaqMan®assaybasedonFAM™andHEX™labeledprobes.

RecommendedUse/Scope

Canbeusedinresearchandindustryfordetectionofmeat/animalbasedcontaminationsofveganfoods.Forresearchuseonly!Donotuseforclinicaldiagnosticorfor testingofpatientsamples.

PackageSizes

• Cat.Nr.12-05-02525Tests
  Cat.Nr.12-05-100100Tests

ResultEvaluation

Cyclerbased,real-timePCR

Sensitivity

Downto0.1%ofgenomicmeattargetDNAinaveganfoodtypebackground.

Content

Primersets,nucleotides,internalamplificationcontrolandpolymeraseatoptimizedconcentrationslyophilizedinonetube,rehydrationbuffer,lyophilizedpositivecontrol,PCRgradewater.

SampleRequirements

IsolatedtotalDNAfrompotentiallycontaminatedveganfoodservesasstartingmaterial.DNAcanbeextractedfromsamplematerialusingDNAextraktionkitse.g.ExtractNow™VeganControl(Cat.No.607-1050)orusermethod.

RequiredConsumables

1.5mlreactiontubes,PCRreactiontubes,sterilefiltertips

RequiredLabDevices

qPCRcyclerwithFAM™andHEX™filter,variablemicroliterPipettes,benchtopcentrifuge

Timetoresult

Approx.150minutes

ShelfLifeandStorage

Componentscanbestoredat+2to+8°Cforatleast12months.Afterrehydratisationthereagentsmustbestoredat-18°C.