TheAquaScreen®qPCRDetectionkitseriesisspecificallydesignedforthequantitativedetectionofcommonwaterpathogenssuchasLegionella,Pseudomonas,andE.coli.TheAquaScreen®approachincludessamplepreparationusingtheAquaScreen®FastExtractkitandvariousqPCRdetectionkits.Incontrasttotime-consumingcellculturemethods,theAquaScreen®approachneedslessthansixhoursincludingsamplepreparationandqPCRtoreliablydetectwaterpathogens.Inaddition,ourqPCRassayissuperiorintermsofsensitivityandspecificityastheassayonlydetectstherespectivespecies.TheAquaScreen®qPCRassayisinsensitivetocontaminationwithotherbacterialgenera.Also,theassay’srobustnessisunsurpassedwithlineardetectionupto3x10⁶particlespersample.

• ➘AquaScreen®Legionellapneumophila
• ➘AquaScreen®Legionellaspecies
• ➘AquaScreen®Pseudomonasaeruginosa
• ➘AquaScreen®Escherichiacoli

AquaScreen®Legionellapneumophila

Features

TypeofPCR

Quantitativereal-timePCR(qPCR)

Description

TheAquaScreen®LegionellapneumophilaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellapneumophilainwaterssamples.TheusedprimersetisspecificforasegmentofthemipregionoftheLegionellapneumophilagenome.

ScopeofApplication

ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.

KitComponents

Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater

PackageSizes

• Cat.No.34-202525reactions
  Cat.No.34-2100100reactions
  Cat.No.34-2250250reactions

RequiredConsumables

PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).

RequiredLabEquipment

qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips

Storage

Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow-18°C.

Compliance

AFNORXPT90-471andISO/TS12869:2012

AquaScreen®Legionellaspecies

Features

TypeofPCR

Quantitativereal-timePCR(qPCR)

Description

TheAquaScreen®LegionellaspeciesqPCRDetectionKitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofLegionellainwaterssamples.Thesuppliedprimersetisspecificforabroadrangeoflegionellaspecies,butdoesnotdetectotherwaterbornebacteriaasrequiredbyISO/TS12869:2012.

ScopeofApplication

ApplicableforwatertestingasdescribedinISO/TS12869:2012,inresearchandindustryforQAtestingofprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.

KitComponents

Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater

PackageSizes

• Cat.No.33-202525reactions
  Cat.No.33-2100100reactions
  Cat.No.33-2250250reactions

RequiredConsumables

PCRreactiontubes
Optional:ForquantificationwerecommendourLegionellapneumophilaPCRQuantificationStandard(Cat.No.52-0101).

RequiredLabEquipment

qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips

Storage

Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow        -18°C.

Compliance

AFNORXPT90-471andISO/TS12869:2012

AquaScreen®Pseudomonasaeruginosa

Features

TypeofPCR

Quantitativereal-timePCR(qPCR)

Description

TheAquaScreen®PseudomonasaeruginosaqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofPseudomonasaeruginosainwaterssamples.

ScopeofApplication

ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.

KitComponents

Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater

PackageSizes

• Cat.No.34-602525reactions
  Cat.No.34-6100100reactions
  Cat.No.34-6250250reactions

RequiredConsumables

PCRreactiontubes
Optional:ForquantificationwerecommendourPseudomonasaeruginosaPCRQuantificationStandard(Cat.No.52-0071).

RequiredLabEquipment

qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips

Storage

Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow        -18°C.

AquaScreen®Escherichiacoli

Features

TypeofPCR

Quantitativereal-timePCR(qPCR)

Description

TheAquaScreen®EscherichiacoliqPCRDetectionkitisusedforDNAsamplespreparedwiththeAquaScreen®FastExtractkitforquantificationofEscherichiacoliinwaterssamples.

ScopeofApplication

ApplicableinresearchandindustryforQAtestingofhouseholdandprocesswater.Notrecommendedforclinicaldiagnostics,testingofhumansamplesorpharmaceuticalproducts.

KitComponents

Freeze-driedPCRmixwithprimer,probes,nucleotides,andpolymerase
RehydrationBuffer
Freeze-driedPositiveControlDNA
Freeze-driedInternalAmplificationControl
PCR-gradeWater

PackageSizes

• Cat.No.34-702525reactions
  Cat.No.34-7100100reactions
  Cat.No.34-7250250reactions

RequiredConsumables

PCRreactiontubes
Optional:ForquantificationwerecommendourE.coliPCRQuantificationStandard(Cat.No.52-0083).

RequiredLabEquipment

qPCRcyclerwithfiltersetsforFAM™andROX™
Pipetsandfiltertips

Storage

Theunopenedkitshouldbestoredat+2to+8°C.Afterrehydrationthereagentsmustbestoredbelow            -18°C.