Mynox®GoldrepresentsthefurtherdevelopmentoftheclassicalMynox®.Mynox®GoldisacombinationofastandardantibioticandtheBIOLOGicalMynox®reagent.Oneapplicationcomprisesofastartertreatmentthateliminatesmostofthemycoplasmaparticleswithoutharmingthecells.Themaintreatmentkillsallremainingmycoplasma.

Benefits

Nocytotoxicity–cytotoxicityisbasicallynotdetectableformostcelltypes.Mostcelllinesdonotshowanychangesinmorphologyduringtreatment.

Lowresistancerisk–duetothecombinationofanantibioticandareagentshowingabiophysicalmodeofactiondirectlyeliminatingthemycoplasma,formationofresistancesagainstMynox®Goldismostunlikely.

Highlyeffective–theratioforapermanenterADIcationiscloseto100%.

RecommendedUse/Scope

Mynox®Goldisintendedforresearchuseonly.Notapplicableforclinicaltreatment.NotapplicableforthedirecttreatmentofenvelopedvirusesorcellsharboringChlamydiaorotherbacteriaashostsystems.Theantibioticportionoftheproductmightaffecttheintegratedmicroorganisms.

KitComponents

1applicationofMynox®Goldcontains1vialforthestartertreatmentand3vialsforthemaintreatment.Eachvialcontains500µlasasterile,ready-to-usesolution.

PackageSizes

1applicationcanbeusedtocureonecellculturepermanentlyfrommycoplasmaburden.

• Cat.No.10-02012applications
  Cat.No.10-05015applications
  Cat.No.10-100110applications

ResultEvaluation

Eliminationsuccessshouldbeverifiedwithasuitablemycoplasmadetectionsystem,e.g.MinervaBiolabs’sVenor®GeMMycoplasmaPCRDetectionKits.

RequiredConsumables

6-welltissuecultureplateorsmalltissueculturepetridish(6mmØ)

Regularcellcultureplastics

RequiredLabDevices

Regularcellcultureequipment(incubator,centrifuge,Pipettes,etc.)

ShelfLifeandStorage

Kitcomponentsaremaintainableat+2to+8°Cforatleast6months.

Fig.CytotoxicityofMynox®Goldoncontaminatedcells.Adherentcelllines(Vero,ML/SMDBKandRK13)weretreatedwithMynox®Goldaccordingtotheprotocol4daysofincubation.ThecultureswereinactivatedandstainedasdescribedbyFlick&Gifford.Untreatedcultureshadbeenusedasreference(100%).